Nobel Saridun Klik erreakzioaren baliagarritasuna konposatu biologikoak eransteko polimero gainazalean

Abstract

Azken urteetan, hezur edo artikulazio akastunen funtzioa berrezartzeko inplante kirurgikoak erabiliak izan ohi dira. Halaber, azken urteetan inplanteen osteointegrazio ona bermatzen duten materialak lortzea erronka zientifikoa bihurtu da. Izan ere, biomaterialaren gainazalaren propietate fisiko kimikoek pazientearen erantzuna modulatzen dute. Hori dela eta, funtsezkoa da aktibitate antiinflamatorioa eta bakterioen aurkako propietateak dituzten inplanteak diseinatzea. Azken hamarkadan Klik kimika aukera interesgarria bilakatu da biomolekula konplexuak material desberdinen gainazaletara kobalenteki lotzeko. Lan honetan biomedikuntzan interesa duen molekula organiko baten inmobilizazio kobalentea deskribatzen da poliazido L-laktikoa (PLLA) polimeroa gainestaldura gisa erabiliz. Proposatutako metodologiaren berrespena Ultramore-ikuskor (UM-Ikus), mikroskopia fluoreszentea, X izpien espektroskopia fotoelektronikoa (XPS) eta angelu kontaktuaren bidez balidatu da.Surgical implants are usually employed to restore the function of affected joints or bones. In recent years, obtaining materials that guarantee good implant osseointegration has become a scientific challenge. In fact, the physico-chemical properties of the surface of the biomaterial play a keyrole in patience response. Therefore, it is essential to design implants with anti-inflammatory activity and antibacterial properties. In the last decade, click chemistry has emerged as the method of choice in order to bind covalently complex biomolecules to the surface of diverse materials. This work describes the covalent immobilization of an organic molecule with interest in biomedicine using Poly-L-Lactic acid (PLLA) polymer as a coating. The validation of the proposed methodology has been assured with different techniques: UV-Vis, fluorescence microscopy, X-ray photoelectron spectroscopy (XPS) and water contact angle.