Morfinak H3K27me3 histonaren antolamenduan sortutako eraldaketak inpronta genomikodun geneen adierazpenean duen eragin zuzenaren egiaztapena, ChIP-seq teknikaren bidez
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Abstract
Kanpo faktore edo estimuluek banakoan duten eragina epigenetikaren bitartez aztertzen da. Aldaketa epigenetikoak ez dira DNA-ren sekuentzian ematen diren mutazioak, DNA-ri itsasten zaizkion molekula kimiko batzuk baizik, geneen adierazpenaren erregulazioan eragina izan dezaketenak. Kanpo faktoreek banako helduan aldaketa fisiologiko ugari sortzen dituztela erakutsi dute ikerketa ezberdinek. Baina pentsa dezagun nolako eragina izango luketen kanpo estimuluek fetuan eta zein ondorio ekarriko lituzke fetuaren garapen prozesuan eta ondorioz banakoan. Lan honen helburua, kanpo estimulua den morfinak, saguaren zelula ametan sortzen dituen aldaketa epigenetikoak aztertzea da, zehazki, errepresio marka bat den histona batean eta genoman zehar berari lotuta agertzen zaizkion geneetan.
Epigenetics is the study of how environmental factors or external stimuli can affect the individual. Epigenetic modifications don’t involve changes in the underlying DNA sequence or mutations; in fact, they are small biochemical molecules that stick to the DNA, regulating the expression of different genes. In the last years, lots of research groups have described that environmental factors have direct impact in physiological traits of adult individuals. But what do we know about the effect of the external stimuli in the fetus, and the consequences that those stimuli can produce in the early stages of the developmental process? The aim of this research is to analyze the epigenetic modifications caused by morphine in mouse embryonic stem cells, more specifically, we have focused the study on a repressive mark histone and the genes attached to this histone throughout the genome.
Epigenetics is the study of how environmental factors or external stimuli can affect the individual. Epigenetic modifications don’t involve changes in the underlying DNA sequence or mutations; in fact, they are small biochemical molecules that stick to the DNA, regulating the expression of different genes. In the last years, lots of research groups have described that environmental factors have direct impact in physiological traits of adult individuals. But what do we know about the effect of the external stimuli in the fetus, and the consequences that those stimuli can produce in the early stages of the developmental process? The aim of this research is to analyze the epigenetic modifications caused by morphine in mouse embryonic stem cells, more specifically, we have focused the study on a repressive mark histone and the genes attached to this histone throughout the genome.
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aldaketa epigenetikoak, H3K27me3 histona, inpronta genomikoa duten geneak, morfina., epigenetic modifications, histone H3K27me3, imprinted genes, morphine.