RNA zirkular berrien bilaketak atzeranzko moztu-itsastearen kontserbazio altua frogatu du ardian

Abstract

RNA zirkularrak (circRNAk) modu kobalentean itxitako RNA ez-kodetzaile zirkularrak dira. Duten egitura dela eta, circRNAk gaixotasunen biomarkatzaile gisa erabiltzeko aproposak dira. Molekula mota hauek garrantzi zientifikoa badute ere, animalia ez-ereduetan ikerketa garatu gabe dago oraindik. Lan honetan bi ehun desberdinen RNA-seq datuak aztertu dira ardiaren circRNAk identifikatzeko. Garun azalean eta odol-zelula mononuklearretan 2510 eta 3403 circRNA detektatu dira, horietatik 1379 guztiz berriak. Harrigarria da detektatu diren circRNA molekulen moztu-itsasteen %63a gizakiarekiko guztiz homologoak direla. Gainera, gizakian ikusi den bezala, emaitzek CDR1-AS circRNA ardiaren garunean dituen funtzioak babesten dituzte.

Circular RNAs (circRNAs) are covalently closed circular non-coding RNAs. Due to their structure, circRNAs are good candidates for disease biomarkers. Despite the scientific relevance of these molecules, the study of circRNAs in non-model organisms is still in its infancy. Here, we analyse RNA-seq data to identify circRNAs from two sheep tissues: peripheral blood mononuclear cells (PBMCs) and parietal lobe cortex. Out of 2510 and 3403 circRNAs detected in parietal lobe cortex and in PBMCs, a total of 1379 novel circRNAs were discovered. Remarkably, around 63% of all detected circRNAs were found to be completely homologous to a circRNA annotated in human. Besides, our results support the miRNA sponge function of CDR1-AS in sheep brain, as reported in human.