Efektuei Bideratutako Analisia ospitaleko efluenteetan disruptore endokrino potentzialen detekziorako legamia bidezko in-vitro biosaioaz baliatuz

Abstract

Ospitale efluenteak urgaineratutako kutsatzaileen ingurumeneko agerpenaren erantzule nagusietako bat kontsideratzen dira izaten dituzten farmako eta disruptore endokrino kontzentrazio altuak kontuan izanda. Lan honetan, efektuei bideratutako analisi (EDA) estrategia erabiltzen da bizkaiko ospitale bateko efluenteko aktibitate estrogenikoaren erantzuleak izan daitezkeen konposatuak identifikatzeko asmoz, zatikatze teknikak, legamia bidezko in-vitro biosaio bat eta susmagarrien analisia konbinatuz. Aktibitatea erakutsi duten frakzioetan detektatu diren estrogenizitatearen hautagaien artean bibliografian disruptore endokrino bezala nabarmendutako zenbait konposatu detektatu dira, hala nola, A bisfenola (1452 ng/L), butilparabenoa (37 ng/L), diurona (3.2 ng/L) eta progesterona (52 ng/L).

Hospital effluents contribute to the occurrence of emerging contaminants in the environment due mainly to their high load of pharmaceutical active compounds and some endocrine disrupting compounds (EDCs). In this study, an effect-directed analysis (EDA) approach has been performed in order to identify the compounds potentially responsible of the endocrine disruption activity in a hospital effluent from Biscay combining fractionation techniques, a recombinant yeast (A-YES) in-vitro bioassay and suspect screening approach. Among the potential candidates for the estrogenic effects of the bioactive fractions, different compounds with known estrogenic activity were detected, including, bisphenol A (1452 ng/L), butylparaben (37 ng/L), diuron (3.2 ng/L) and progesterone (52 ng/L).